High-performance liquid chromatography (HPLC) is a technique used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different retention times for the components and leading to the separation of the components as they flow out the column. Operating parameters such as mobile phase composition, stationary phase chemistry, flow rate and temperature provide a wide range of separation applications. Maximum operating pressures up to around 4500 psi are common (dependent upon column chemistry).

Ultra high performance liquid chromatography (UPLC) uses particle sizes smaller than those used in HPLC applications. Maximum operating pressures up to 15,000 psi provides better separations and faster analysis times.

Numerous detection techniques are available. The most common is UV detection but others include Refractive Index (RI), Conductivity, Fluorescence and Mass Spectrometry.


Current Methods:
CA413 Method Validation for HPLC Assays
CA416 Peak Purity Analysis using the Waters PDA
CA540 Out of Specification Results (OOS) for HPLC Assays
CA14001 Hydroquinone by HPLC
CA14007 Benzoyl Peroxide Impurites
CA14009 Sodium MonoFluorophosphate and Fluoride by Ion Chromatography
CA14010 Phenethylresorcinol by HPLC
CA14011 Lidocaine Hydrochloride by HPLC
CA14012 Dibucaine Ointment By HPLC
CA14014 Benzoyl Peroxide Products by HPLC
CA14016 Kojic Acid & Arbutin by HPLC
CA14017 Benzocaine by HPLC
CA14019 HPLC Analysis of Phenonip
CA14021 Clotrimazole Products by HPLC
CA14026 Hydrocortisone by HPLC
CA14031 HPLC Analysis of Benzethonium Chloride
CA14033 Ketaconazole Products by HPLC
CA14034 Hydrocortisone Acetate by HPLC
CA14040 HPLC Analysis of Miconazole Nitrate
CA14041 HPLC Analysis of Mikrokill COS RL 18
CA14042 Aminophylline by HPLC
CA14043 HPLC Analysis of Phenoxyethanol, Sorbate, Benzoate & Dehydroacetate
CA14045 Copper Peptides by HPLC
CA14047 HPLC Analysis of Methylisothiazolinone
CA14048 Pramoxine Hydrochloride by HPLC
CA14054 Tolnaftate by HPLC
CA14055 Petrolatum Content by GPC
CA14060 Azelaic Acid by HPLC
CA14064 Phenylephrine HCl in Suppositories by HPLC
CA14065 Tetracaine by HPLC
CA14067 Cetyl Pyridinium Chloride by HPLC
CA14069 Glycerol by Ion Exclusion HPLC
CA14077 Sorbitol by HPLC
CA14080 Formaldehyde by HPLC
CA15001 Phenylbenzimidazole Sulfonic Acid (Ensulizole) by HPLC
CA15002 Sodium Sulfacetamide by HPLC
CA15024 Sunscreens by HPLC
CA15025 HPLC Analysis of Benzalkonium Chloride
CA15026 Dimethicone by GPC
CA15027 Tinsorb S / Escalol S by HPLC
CA15028 Trolamine (TEA) Salicylate by HPLC
CA15029 Chlorhexidine Gluconate by HPLC
CA15031 Progesterone by HPLC
CA15033 Organic Acids by Ion Exclusion HPLC
CA15035 Progesterone & Vitamin E Acetate by HPLC
CA15037 Diphenhydramine Hydrochloride by HPLC
CA15039 Salicylic Acid by HPLC
CA15040 Collagen by HPLC
CA15042 Chondroitin by GPC
CA15043 Hyaluronic Acid Analysis by GPC
CA15045 Crystal Violet by HPLC
CA15046 p- Chloroxylenol (PCMX) by HPLC
CA15051 Finasteride by HPLC
CA15062 Benzyl Alcohol & Parabens by HPLC
CA15071 Acetaminophen by HPLC
CA15083 Allantoin by HPLC
CA15084 Pyrethrins, Piperonly Butoxide and Permethrins by HPLC
CA17001 Minoxidil by HPLC
CA17004 Carotene by HPLC
CA17006 Caffeine by HPLC
CA17007 Melatonin by HPLC
CA17013 Urea by HPLC
CA17014 Adenosine by HPLC
CA17016 Glucosamine by HPLC
CA17017 Capsaicinoids by HPLC
CA17021 Hydroxycitric Acid by Ion Exclusion HPLC
CA17027 Aloe Polysaccharides by GPC
CA17029 HPLC Assay of Aloin
CA17032 Emodin and Aloe Emodin by HPLC
CA17043 Niacinamide Raw Material by HPLC
CA17047 Epigallacatechin Galate by HPLC
CA17055 Sodium Thioctate by HPLC
CA17065 Sodium Ascorbyl Phosphate by HPLC
CA17066 Panthenol by HPLC
CA17067 Molecular Weight by GPC
CA17072 Mesembrine Alkaloids by HPLC
CA17077 L-Theanine by HPLC
CA17079 GABA by HPLC
CA17084 Biotin Raw Material By HPLC
CA17085 Calcium Pantothenate by HPLC
CA17089 Vitamins D2 & D3 by HPLC
CA17090 Sulfur by HPLC
CA17094 Oil Soluble Vitamins by HPLC
CA17095 Water Soluble Vitamins By HPLC
CA17096 Riboflavin, Biotin, Vitamin B-12 by HPLC
CA17098 Thiamine by HPLC
CA17099 Riboflavin Phosphate Purity by Fluorimetry
CA18002 Amino Acids by UHPLC
CA18003 Sesamin and Sesamolin by UHPLC
CA18009 L-Carnitine by HPLC
CA25001 Kava Lactones and Flavokawains by HPLC
CA25004 Riboflavin Phosphate & Vitamin B12